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We did this by using a Gilson Respirometer and samples of Elodea plant placed in H2O and NaHCO 3 solutions—both of which were operated under lighte d and darkened external conditions—to measure the consumption of CO 2 during photosynthesis and the production of CO 2 during respiration. Censullo Monica Mendoza Saul Longoria... Inhibition Of Direct Light Affects Elodea 's Co2 Production During Photosynthesis. A. Elodea in AQUARIUM Water 1. When water plants like Elodea are photosynthesizing, it … During photosynthesis, plants are using the energy of the sun to build molecules which effectively store this energy (glucose). More advanced students can dive deeper into understanding the plant life cycle, chemistry, and biology in our photosynthesis worksheets. algae Elodea. Change the conditions of photosynthesis by altering light intensity and carbon dioxide amount, and determine the effects on the photosynthesis rate. Photosynthesis Experiments are centered on the investigation of two areas pertaining to photosynthesis: factors that affect the photosynthetic process; and cellular structures or substances involved in photosynthesis. Photosynthesis - Resources: Demonstrating oxygen formation during photosynthesis can be a tricky process. Tube 3 will be the Elodea in the light. Photosynthesis, the process by which green plants and certain other organisms transform light energy into chemical energy. Using forceps remove a small leaf from the tip of an Elodea plant and lay … Photosynthesis-Elodea Lab Photosynthesis is the process by which plants take carbon dioxide from the atmosphere, add water, and use the energy of sunlight to produce sugar. 3. However both ways will demonstrate how photosynthesis produces oxygen, and cellular respiration uses oxygen and create Carbon dioxide. NARRATOR: The electrons lost from chlorophyll during photosynthesis have to be replaced. Light Dark. One common way is to gather bubbles of gas given off by an aquatic plant. Students learn a simple technique for quantifying the amount of photosynthesis that occurs in a given period of time, using a common water plant (Elodea). Note: Before beginning this activity, use scissors to make an Elodea cutting for each group, cutting the stems in between the leaf nodes. In this experiment I use a plant called Elodea (pond weed). This test can be done with and without snails. Test Tube Treatment. Standard error: 0.84 for RuDP Carboxylase and 0.72 PEP Carboxylase. Prepare a wet mount of an Elodea leaf with aquarium water. 1. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a by-product. Count bubbles of oxygen gas given off by elodea to determine the rate of photosynthesis. Photosynthesis and respiration; Description Therefore in the presence of light, Elodeawill take up CO2, causing the concentration of CO2 in its surroundings to drop. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Bio 2 Elodea Lab: Photosynthesis and Cellular Respiration Name_____per____ Objectives: to compare the reactions of photosynthesis and cellular respiration. Add 1-2 drops of bromothymol blue to 15mL of water in a 25 ml graduated cylinder. O. CO. 2 (needed for photosynthesis) and . However, Cabomba is a more reliable plant and much more effective than the traditional Elodea. Tube 1 will be the control (no Elodea) Tube 2 will be the Elodea in the dark. Through experiments, students determine that carbon dioxide, light, and chlorophyll are three of the necessary components of photosynthesis. More specifically, you will learn how the amount of light affects photosynthesis. Have at least 7-9 lab stations set up in the room with lamps. 2) Crush the end of the stem at the site of incision gently. Plants do this by splitting water molecules and using electrons from hydrogen, leaving oxygen as waste product. Part 3: Rate of Photosynthesis Lab Investigation. Elodea 27.9 + 2.2 14.21 + 2.5 1.96 38.1 0.72 2.5 Spinacia 66.6 11.1 6.00 69.3 2.10 2.9 Zea 31.5 147.9 0.21 130.5 2.31 3.44 1Standard deviation. The Floating Leaf Disk Assay for Investigating Photosynthesis Introduction: Trying to find a good, quantitative procedure that students can use for exploring photosynthesis is a challenge. To demonstrate that carbon dioxide is produced during respiration. What is the photosynthesis equation? The rate of photosynthesis varies greatly with changes in wavelengths of light. To observe the rate of photosynthesis under different light conditions. 2. Elodea & Photosynthesis Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize nutrients from carbon dioxide and water. Originally from Brazil, E. densa has spread to temperate regions on all of the continents except Antarctica. They use this technique to compare the amounts of photosynthesis that occur under conditions of low and high light levels. Bromothymol blue is an indicator solution to those used to test for food nutrients. Click: Elodea and snails; Just Elodea; Question s 5. 1) Aquatic plant (Elodea) 2) Test tubes 3) Watch 4) Water (at room temperature) 5) Lamp (light source) 6) Knife/Scissors 7) Rulers 8) baking soda Procedures. During photosynthesis in green plants, light energy is captured and used to convert water, carbon dioxide, and minerals into oxygen and energy-rich organic compounds. Students will have to utilize keen observation skills to count the number of bubbles that form in the different settings. To do this, use a soaked “Q-Tip” to place a drop of aquarium water towards one end of the slide. It starts as a blue solution in water, and turns yellow in the presence of carbon dioxide. Bromothymol Blue (which acts as an indicator to show if photosynthesis is occurring). 2 An experiment that uses aquatic plants shows a student that plants need light for the photosynthesis process. ©2008 CIBT Photosynthesis and Respiration in Elodea – Student Section Page 2 Purpose: Your lab group is asked to design, execute, and analyze an experiment that tries to accomplish the following two tasks: Task A. CO2 concentration (BTB color indicator number) 0 6 7 8. Photosynthesis is a process by which phototrophs convert light energy into chemical energy, which is later used to fuel cellular activities. Phosphoglyceric acid and sugars account for 27% of the label during similar exposures. on Photosynthesis of Elodea. Students will record their data which will be used to conclude whether carbon dioxide was consumed or released by the elodea. No matter your students' level, we have an activity to stretch their scientific knowledge. Little learners can explore the basic ingredients of plant life by tracing, coloring, and completing diagrams. In this practical students will be able to see photosynthesis and cellular respiration in action. in photosynthesis per unit time is much greater than the amount generatedby respiration over the same time period (about 10-30 times greater.) In this inquiry lab, students design and conduct simple experiments using elodea and Bromothymol blue to determine whether plants consume or release carbon dioxide in the process of photosynthesis. Experiments to determine the early labeled photosynthetic products in Elodea canadensis show that after 2 seconds of exposure to NaH 14 CO 3, 45% of the 14 C incorporated is located in malate and aspartate. The solution containing Elodea maintained in the dark did not undergo a color change as seen in the Elodea in the light. ATP and hydrogen ions and electrons are used in the second stage of photosynthesis, the ‘light-independent reactions’. Photosynthesis, derived from the Greek words photo, meaning "light," and synthesis "putting together," is a process used by plants and some bacteria to harness the energy from sunlight to convert water and carbon dioxide to produce sugar (glucose) and oxygen. The chemical energy is stored in the form of sugars, which are created from water and carbon dioxide. Known for its bright green color and leafy stems, E. densais an excellent oxygenating plant, making it perfect for use in fresh… 1) Cut a segment of the Elodea plant approximately 8cm with scissors. Assemble the equipment needed to measure the rate of photosynthesis in elodea (water plant). Pour 25 mL of the bromothymol blue + water solution into each of the three test tubes. Cover Tube 1 with tin foil (there is no Elodea in Tube 1) and place it in the test tube rack. Add a 7 cm piece of Elodea to Tube 2. Photosynthesis and Respiration in Elodea Background Concepts: Plants can carry out both photosynthesis and respiration simultaneously. // Provide alternate content for browsers that do not support scripting // or for those that have scripting disabled. Bromothymol blue works because it is able to detect the presence of . Photosynthesis, respiration, and release of organic carbon were examined using 14 C in the freshwater angiosperm Elodea canadensis Michx. Elodea is a leafy flowering plant commonly found in aquariums. to investigate ecophysiological implications of recent biochemical evidence for significant levels of C 4 photosynthesis in this plant, a pathway normally associated with low photorespiration and high photosynthetic efficiency. Please check your test tubes and use the data to complete your Data Table and Graph on the back of your Lab Sheet. Photosynthesis of Elodea Inhibition Of Direct Light Affects Elodea 's Co2 Production During Photosynthesis. Light's colour is determined by its wavelength of light, and thus it is possible to devise an experiment to determine which wavelengths of light are most productive for photosynthesis than others. During the ‘light-independent reactions’, carbon dioxide, taken in from the air, is combined with hydrogen and ATP to form a range of organic molecules for the plant. In order to do this, you will use an . The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable. Its leaves are thin and transparent; most leaves are only two cell layers thick. Egeria densa, also known as Elodea densa because of its previously incorrect classification in the Elodea genus, is the most popular aquatic plant used to teach photosynthesis in the classroom. Instruct students to work in groups of 2-3 students to conduct the Photosynthesis in Elodea lab. Demonstrate that environmental CO2 is used during photosynthesis in Elodea. 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